(-)-Epigallocatechin-3-gallate(EGCG)가 타액선 암세포의 제1형 교원질 부착 및 이동에 미치는 영향
Effect of (-)-Epigallocatechin-3-gallate (EGCG) on SGT Cell Adhesion and Migration to Type Ⅰ Collagen Treatment
윤효은, 윤정훈, 안상건,
소속 상세정보
윤효은 ( Yoon Hyo-Eun ) - 조선대학교 치과대학 병리학교실
윤정훈 ( Yoon Jung-Hoon ) - 조선대학교 치과대학 병리학교실
안상건 ( Ahn Sang-Gun ) - 조선대학교 치과대학 병리학교실
KMID : 0829220110350030147
Abstract
EGCG has inhibitory effect on a variety of cancers by inducing apoptosis and cell cycle arrest or inhibiting angiogenesis and metastasis. EGCG has been found to induce apoptosis in salivary gland carcinoma cells. But the potential anti-invasive effect of EGCG in salivary gland cancer has not been studied yet. The aim of this study is to evaluate the effect of EGCG on salivary gland adenocarcinoma SGT cell adhesion and migration to Type Ⅰ collagen treatment. Western blot, adhesion and migration assay were performed to evaluate the impacts of EGCG on the expression of MMP-2/-9 and its upstream signaling molecules after treatment of type Ⅰ collagen. SGT cell adhesion to type Ⅰ collagen is significantly suppressed by EGCG. EGCG decreased expression of β1 integrin, phosphorylation of FAK, MMP-2/-9 compared with type I collagen treatment. In addition, EGCG inhibited the migration of SGT cells treated with type Ⅰ collagen. These results suggest that EGCG could effectively inhibit the invasion and migration of human SGT cells by downregulating the expression of β1 integrin and MMP-2/-9 and phosphorylation of FAK, Akt, and Erk. Adhesion and migration to type Ⅰ collagen of SGT cells can be influenced through EGCG.
키워드
EGCG; Invasion; Migration; Salivary gland cancer; Type Ⅰ collagen; β1 integrin signaling
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